Microbial cell surface display technology holds great strategic potential for expressing heterologous proteins or polypeptides with a distinct function on the surface of microbial cells. To gain amenable cell surface exposure, the passenger protein could be fused with various anchoring motifs (carrier protein), which are usually cell-surface proteins or their fragments. Up to the characteristics of passenger and carrier proteins, this strategy can be carried out using different fusion methods, such as C-terminal fusion, N-terminal fusion or sandwich fusion. Creative Biogene provides various cell-surface display systems for the successful display of a target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif.
Microbial cell surface display technology holds great strategic potential for expressing heterologous proteins or polypeptides with a distinct function on the surface of microbial cells. To gain amenable cell surface exposure, the passenger protein could be fused with various anchoring motifs (carrier protein), which are usually cell-surface proteins or their fragments. Up to the characteristics of passenger and carrier proteins, this strategy can be carried out using different fusion methods, such as C-terminal fusion, N-terminal fusion or sandwich fusion. Creative Biogene provides various cell-surface display systems for the successful display of a target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif.
Surface display systems developed for bacteriophages (phage)
Phage display has been used as a high-throughput screening technology which allows the presentation of peptide and protein libraries on the surface of phage with many remarkable advantages.
Surface display systems developed for Gram-negative bacteria
For Gram-negative bacteria, including E. coli, the fragility of outer membrane caused by the display of proteins can be a problem. Nevertheless, E. coli is still an attractive host because it has the advantage of high transformation efficiencies and the availability genetic tools and mutant strains.
Optional anchoring motifs for the enriched display of a target of interest
• Outer membrane protein：OmpA/OprF/LamB
• Lipoprotein：PAL/TraT/Opr1
• Passenger protein：AIDA-I
• S-layer protein
• Ice nucleation protein (INP)
• Pullulanase
Surface display systems developed for Gram-positive bacteria
For Gram-positive bacteria surface display, Bacillus and Staphylococcus strains have been used most often. Gram-positive bacteria seem to be more suitable for whole-cell catalysts and whole-cell adsorbents owing to the rigid structure of outer cell walls. Meanwhile, proteins are presented on the surfaces only through a single membrane layer, avoiding negative influences during the traverse through the periplasmic space and the integration on the outer membrane.
